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wnt signaling pathway specific inhibitor lf3  (Cayman Chemical)

 
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    Cayman Chemical wnt signaling pathway specific inhibitor lf3
    Effect of AGE on the β-defensin 4 production and canonical Wnt pathway in GE1 mouse gingival epithelial cells. (A) Cells were treated with AGE at the indicated concentrations (0.25-2 mg/ml) for 24 h. The amount of β-defensin 4 protein secreted into the culture medium was determined using an ELISA. The graph shows the concentration of β-defensin 4 in the medium. Data are presented as the mean ± standard deviation (n=6). * P<0.05 (Dunnett's test). (B) Cells were treated with AGE (2 mg/ml) for the indicated duration (1-24 h). The protein level of β-catenin in the nucleus was analyzed by western blotting. Images show representative data of western blotting for β-catenin with lamin B1 as the internal control. The graph shows the level of β-catenin protein normalized to that of lamin B1. Data are presented as the mean ± standard deviation (n=3-7). * P<0.05 (Mann-Whitney U test). (C) Cells were treated with AGE for 24 h in the presence or absence of <t>LF3</t> (30 µM; 30-min pretreatment), an inhibitor of β-catenin in the canonical Wnt pathway. The amount of β-defensin 4 protein secreted into the culture medium was determined using an ELISA. The graph shows the concentration of β-defensin 4 in the medium. Data are presented as the mean ± standard deviation (n=4). * P<0.05, ** P<0.01 (Holm-Bonferroni test). A, AGE; AGE, aged garlic extract; C, control.
    Wnt Signaling Pathway Specific Inhibitor Lf3, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wnt signaling pathway specific inhibitor lf3/product/Cayman Chemical
    Average 90 stars, based on 1 article reviews
    wnt signaling pathway specific inhibitor lf3 - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Aged garlic extract enhances the production of β‑defensin 4 via activation of the Wnt/β‑catenin pathway in mouse gingiva"

    Article Title: Aged garlic extract enhances the production of β‑defensin 4 via activation of the Wnt/β‑catenin pathway in mouse gingiva

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2024.12791

    Effect of AGE on the β-defensin 4 production and canonical Wnt pathway in GE1 mouse gingival epithelial cells. (A) Cells were treated with AGE at the indicated concentrations (0.25-2 mg/ml) for 24 h. The amount of β-defensin 4 protein secreted into the culture medium was determined using an ELISA. The graph shows the concentration of β-defensin 4 in the medium. Data are presented as the mean ± standard deviation (n=6). * P<0.05 (Dunnett's test). (B) Cells were treated with AGE (2 mg/ml) for the indicated duration (1-24 h). The protein level of β-catenin in the nucleus was analyzed by western blotting. Images show representative data of western blotting for β-catenin with lamin B1 as the internal control. The graph shows the level of β-catenin protein normalized to that of lamin B1. Data are presented as the mean ± standard deviation (n=3-7). * P<0.05 (Mann-Whitney U test). (C) Cells were treated with AGE for 24 h in the presence or absence of LF3 (30 µM; 30-min pretreatment), an inhibitor of β-catenin in the canonical Wnt pathway. The amount of β-defensin 4 protein secreted into the culture medium was determined using an ELISA. The graph shows the concentration of β-defensin 4 in the medium. Data are presented as the mean ± standard deviation (n=4). * P<0.05, ** P<0.01 (Holm-Bonferroni test). A, AGE; AGE, aged garlic extract; C, control.
    Figure Legend Snippet: Effect of AGE on the β-defensin 4 production and canonical Wnt pathway in GE1 mouse gingival epithelial cells. (A) Cells were treated with AGE at the indicated concentrations (0.25-2 mg/ml) for 24 h. The amount of β-defensin 4 protein secreted into the culture medium was determined using an ELISA. The graph shows the concentration of β-defensin 4 in the medium. Data are presented as the mean ± standard deviation (n=6). * P<0.05 (Dunnett's test). (B) Cells were treated with AGE (2 mg/ml) for the indicated duration (1-24 h). The protein level of β-catenin in the nucleus was analyzed by western blotting. Images show representative data of western blotting for β-catenin with lamin B1 as the internal control. The graph shows the level of β-catenin protein normalized to that of lamin B1. Data are presented as the mean ± standard deviation (n=3-7). * P<0.05 (Mann-Whitney U test). (C) Cells were treated with AGE for 24 h in the presence or absence of LF3 (30 µM; 30-min pretreatment), an inhibitor of β-catenin in the canonical Wnt pathway. The amount of β-defensin 4 protein secreted into the culture medium was determined using an ELISA. The graph shows the concentration of β-defensin 4 in the medium. Data are presented as the mean ± standard deviation (n=4). * P<0.05, ** P<0.01 (Holm-Bonferroni test). A, AGE; AGE, aged garlic extract; C, control.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Concentration Assay, Standard Deviation, Western Blot, Control, MANN-WHITNEY



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    wnt signaling pathway specific inhibitor lf3  (Cayman Chemical)
    90
    Cayman Chemical wnt signaling pathway specific inhibitor lf3
    Effect of AGE on the β-defensin 4 production and canonical Wnt pathway in GE1 mouse gingival epithelial cells. (A) Cells were treated with AGE at the indicated concentrations (0.25-2 mg/ml) for 24 h. The amount of β-defensin 4 protein secreted into the culture medium was determined using an ELISA. The graph shows the concentration of β-defensin 4 in the medium. Data are presented as the mean ± standard deviation (n=6). * P<0.05 (Dunnett's test). (B) Cells were treated with AGE (2 mg/ml) for the indicated duration (1-24 h). The protein level of β-catenin in the nucleus was analyzed by western blotting. Images show representative data of western blotting for β-catenin with lamin B1 as the internal control. The graph shows the level of β-catenin protein normalized to that of lamin B1. Data are presented as the mean ± standard deviation (n=3-7). * P<0.05 (Mann-Whitney U test). (C) Cells were treated with AGE for 24 h in the presence or absence of <t>LF3</t> (30 µM; 30-min pretreatment), an inhibitor of β-catenin in the canonical Wnt pathway. The amount of β-defensin 4 protein secreted into the culture medium was determined using an ELISA. The graph shows the concentration of β-defensin 4 in the medium. Data are presented as the mean ± standard deviation (n=4). * P<0.05, ** P<0.01 (Holm-Bonferroni test). A, AGE; AGE, aged garlic extract; C, control.
    Wnt Signaling Pathway Specific Inhibitor Lf3, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wnt signaling pathway specific inhibitor lf3/product/Cayman Chemical
    Average 90 stars, based on 1 article reviews
    wnt signaling pathway specific inhibitor lf3 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

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    Effect of AGE on the β-defensin 4 production and canonical Wnt pathway in GE1 mouse gingival epithelial cells. (A) Cells were treated with AGE at the indicated concentrations (0.25-2 mg/ml) for 24 h. The amount of β-defensin 4 protein secreted into the culture medium was determined using an ELISA. The graph shows the concentration of β-defensin 4 in the medium. Data are presented as the mean ± standard deviation (n=6). * P<0.05 (Dunnett's test). (B) Cells were treated with AGE (2 mg/ml) for the indicated duration (1-24 h). The protein level of β-catenin in the nucleus was analyzed by western blotting. Images show representative data of western blotting for β-catenin with lamin B1 as the internal control. The graph shows the level of β-catenin protein normalized to that of lamin B1. Data are presented as the mean ± standard deviation (n=3-7). * P<0.05 (Mann-Whitney U test). (C) Cells were treated with AGE for 24 h in the presence or absence of LF3 (30 µM; 30-min pretreatment), an inhibitor of β-catenin in the canonical Wnt pathway. The amount of β-defensin 4 protein secreted into the culture medium was determined using an ELISA. The graph shows the concentration of β-defensin 4 in the medium. Data are presented as the mean ± standard deviation (n=4). * P<0.05, ** P<0.01 (Holm-Bonferroni test). A, AGE; AGE, aged garlic extract; C, control.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Aged garlic extract enhances the production of β‑defensin 4 via activation of the Wnt/β‑catenin pathway in mouse gingiva

    doi: 10.3892/etm.2024.12791

    Figure Lengend Snippet: Effect of AGE on the β-defensin 4 production and canonical Wnt pathway in GE1 mouse gingival epithelial cells. (A) Cells were treated with AGE at the indicated concentrations (0.25-2 mg/ml) for 24 h. The amount of β-defensin 4 protein secreted into the culture medium was determined using an ELISA. The graph shows the concentration of β-defensin 4 in the medium. Data are presented as the mean ± standard deviation (n=6). * P<0.05 (Dunnett's test). (B) Cells were treated with AGE (2 mg/ml) for the indicated duration (1-24 h). The protein level of β-catenin in the nucleus was analyzed by western blotting. Images show representative data of western blotting for β-catenin with lamin B1 as the internal control. The graph shows the level of β-catenin protein normalized to that of lamin B1. Data are presented as the mean ± standard deviation (n=3-7). * P<0.05 (Mann-Whitney U test). (C) Cells were treated with AGE for 24 h in the presence or absence of LF3 (30 µM; 30-min pretreatment), an inhibitor of β-catenin in the canonical Wnt pathway. The amount of β-defensin 4 protein secreted into the culture medium was determined using an ELISA. The graph shows the concentration of β-defensin 4 in the medium. Data are presented as the mean ± standard deviation (n=4). * P<0.05, ** P<0.01 (Holm-Bonferroni test). A, AGE; AGE, aged garlic extract; C, control.

    Article Snippet: A canonical Wnt signaling pathway specific inhibitor LF3 and a glycogen synthase kinase-3 (GSK-3) specific inhibitor 6-bromoindirubin-3'-oxime (BIO) were from Cayman Chemical (Ann Arbor).

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Standard Deviation, Western Blot, Control, MANN-WHITNEY